ProMiFa: Protein Microsequencing Facility
Contact Person: Annapaola Andolfo
The Protein Microsequencing Facility provides different analytical services aimed at structurally characterizing peptides and proteins. The Facility is equipped with analytical HPLC systems, 2D gel electrophoresis apparatus and mass spectrometers. Access to mass spectrometry is a crucial key in the emerging field of proteomics, which is giving a major contribution to the description and understanding of biological processes at the molecular level. Our principal approach for the analysis of proteins is ‘bottom-up’ proteomics, where all proteins are proteolytically digested, producing signature peptides of the original proteins.
The Facility is intended to be a resource for both local and extramural scientific community.
- The tubulin acetyltransferase αTAT1 destabilizes microtubules independently of its acetylation activity. Kalebic N, Martinez C, Perlas E, Hublitz P, Bilbao-Cortes D, Fiedorczuk K, Andolfo A, Heppenstall PA.bMol Cell Biol. 2012 Dec 28. [Epub ahead of print]
- CDKL5 ensures excitatory synapse stability by reinforcing NGL-1-PSD95 interaction in the postsynaptic compartment and is impaired in patient iPSC-derived neurons. Ricciardi S, Ungaro F, Hambrock M, Rademacher N, Stefanelli G, Brambilla D, Sessa A, Magagnotti C, Bachi A, Giarda E, Verpelli C, Kilstrup-Nielsen C, Sala C, Kalscheuer VM, Broccoli V. Nat Cell Biol. 2012 Sep;14(9):911-23. doi: 10.1038/ncb2566. Epub 2012 Aug 26.
- Protein profiling reveals energy metabolism and cytoskeletal protein alterations in LMNA mutation carriers. Magagnotti C, Bachi A, Zerbini G, Fattore E, Fermo I, Riba M, Previtali SC, Ferrari M, Andolfo A*, Benedetti S*. Biochim Biophys Acta. 2012 Jun;1822(6):970-9. doi: 10.1016/j.bbadis.2012.01.014. Epub 2012 Feb 3.