ProMiFa: Protein Microsequencing Facility

Logo ProMiFa

Contact Person: Annapaola Andolfo, PhD

Phone: +39.02.2643.2714 (office)
+39.02.2643.2253/4928 (lab)
Fax: +39.02.2643.6585

The Protein Microsequencing Facility provides different analytical services aimed at structurally characterizing small molecules, peptides and proteins. The Facility is equipped with state-of-the-art mass spectrometers. Access to mass spectrometry is a crucial key in the emerging field of proteomics and metabolomics, which are giving a major contribution to the description and understanding of biological processes at the molecular level.
The Facility is intended to be a resource for both local and extramural scientific community.

Recent Publications:

  • Nebuloni M, Albarello L, Andolfo A, Magagnotti C, Genovese L, Locatelli I, Tonon G, Longhi E, Zerbi P, Allevi R, Podestà A, Puricelli L, Milani P, Soldarini A, Salonia A, Alfano M. Insight on colorectal carcinoma infiltration by studying perilesional extracellular matrixSci Rep. 2016 Mar 4; 6:22522. doi: 10.1038/srep22522.
  • Brunati M, Perucca S, Han L, Cattaneo A, Consolato F, Andolfo A, Schaeffer C, Olinger E, Peng J, Santambrogio S, Perrier R, Li S, Bokhove M, Bachi A, Hummler E, Devuyst O, Wu Q, Jovine L, Rampoldi L. The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin. Elife. 2015 Dec 17;4. pii: e08887. doi: 10.7554/eLife.08887.
  • Barbariga M, Curnis F, Andolfo A, Zanardi A, Lazzaro M, Conti A, Magnani G, Volontè MA, Ferrari L, Comi G, Corti A, Alessio M. Ceruloplasmin functional changes in Parkinson’s disease-cerebrospinal fluid. Mol Neurodegener. 2015 Nov 4; 10: 59. doi: 10.1186/s13024-015-0055-2.
  • D’Addio F, La Rosa S, Maestroni A, Jung P, Orsenigo E, Ben Nasr M, Tezza S, Bassi R, Finzi G, Marando A, Vergani A, Frego R, Albarello L, Andolfo A, Manuguerra R, Viale E, Staudacher C, Corradi D, Batlle E, Breault D, Secchi A, Folli F, Fiorina P. Circulating IGF-I and IGFBP3 levels control human colonic stem cell function and are disrupted in diabetic enteropathy. Cell Stem Cell. 2015 Oct 1;17(4):486-98. doi: 10.1016/j.stem.2015.07.010.
  • Barbariga M, Curnis F, Spitaleri A, Andolfo A, Zucchelli C, Lazzaro M, Magnani G, Musco G, Corti A, Alessio M. Oxidation-induced structural changes of ceruloplasmin foster NGR motif deamidation that promotes integrin binding and signaling. Biol. Chem. 2014 Feb 7; 289(6):3736-48. doi: 10.1074/jbc.M113.520981. Epub 2013 Dec 23.
  • Magagnotti C, Matassa PG, Bachi A, Vendettuoli V, Fermo I, Colnaghi MR, Carletti RM, Mercadante D, Fattore E, Mosca F, Andolfo A. Calcium signaling-related proteins are associated with broncho-pulmonary dysplasia progression. Proteomics. 2013 Dec 6; 94: 401-12. doi: 10.1016/j.jprot.2013.10.007. Epub 2013 Oct 16.
  • Kalebic N, Martinez C, Perlas E, Hublitz P, Bilbao-Cortes D, Fiedorczuk K, Andolfo A, Heppenstall PA. Tubulin acetyltransferase αTAT1 destabilizes microtubules independently of its acetylation activity. Mol Cell Biol. 2013 Mar; 33(6):1114-23. doi: 10.1128/MCB.01044-12. Epub 2012 Dec 28.



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