Advanced Light and Electron Microscopy Bio Imaging Center
Alembic is envisioned as an environment in which biological experimentation requiring electronic or optical imaging will be expedited through the ready access to state-of-the-art instrumentation. Modern biological imaging technology has become sufficiently sophisticated as to require specialized expertise and technology that is often beyond the reach of individual investigators. Nonetheless a broad range of biological investigators will derive significant benefit from access to such avant-garde technology, particularly as we begin the post-genomic era of biomedical research.
Core Facility for Conditional Mutagenesis
The aim of the Core Facility for Conditional Mutagenesis (CFCM) is to offer a centralized service to efficiently produce genetically modified mice for intra and extramural researchers.
CFCM staff generate knock-out, knock-in and conditional mutant mice via both conventional and CRISPR-Cas9 technology. Moreover, the facility performs embryo rederivations, sperm and embryo cryopreservations and isolation of Embryonic Stem cells from murine lines. In addition, CFCM helps researchers to plan experiments, manipulate and genotype mice.
The mice produced at CFCM are Specific Pathogen Free or Specific and Opportunistic Pathogen Free, thereby they can enter any animal house.
Flow cytometry Resource, Advanced Cytometry Technical Applications Laboratory
Located on the Ospedale San Raffaele campus the Flow cytometry Resource and Advanced Cytometry Technical Applications Laboratory (FRACTAL) is a core facility that offers state-of-the-art instrumentation and analysis techniques to the scientific community. In addition to the analytical capabilities of this technique, the core facility offers assisted cell Sorting services for characterizing, separating and purifying populations of particles as diverse as beads, micro-particles and cells.
OMC is a scientific and technological platform aimed at supporting R&D activities related to the validation and use of mouse models of human disease. The facility is rationally designed to ensure a controlled flow of animals from the SPFO vivarium to the areas designed for diagnostic and monitoring procedures. Animals obtained from external sources access such areas directly and can be hosted in clean, ventilated closets for as long as required by the diagnostic protocols. Specialized staff is appointed full time in the various areas and received supports from professionals operating in the hospital, particularly as it pertains to the imaging and comparative pathology activities.
Several modalities allow visualizing organs, tissues, or cells, in living animals. Some imaging modalities are exclusive for preclinical imaging, as the fluorescent and bioluminescent imaging, instead most of them are the same applied in the clinical setting. The Preclinical MRI and US Facility is equipped with a 7T Magnetic Resonance (Bruker; BioSpec 70/30), a dedicated Ultrasound scanner (Vevo 2100; Visualsonic) and a combined Optical – Computed Tomography scanner (IVIS SpectrumCT® Perkin Elmer), therefore as the capability to offer tailored assistance to researchers needing to characterize their small animal models or to perform longitudinal imaging studies. All the modalities are non-invasive and are managed with the collaboration of imaging experts, which may propose the best imaging protocol for each specific task.
Protein Microsequencing Facility
The Protein Microsequencing Facility was established in 2010 at the San Raffaele Scientific Institute.
The facility employs the most advanced mass spectrometry technologies and bioinformatics tools to provide both protein micro-characterization and large scale proteomics services.
Recently, an advanced metabolomics/lipidomics platform has been established.
Excellence Center for High Field Magnetic Resonance